City's U18 side suffered only their second defeat of the season, going down by six goals to nil against Manchester City at Platt Lane.
The hosts fielded a strong side, but used just one overaged player, and the Citizens romped away with the contest.
Manchester City were the divisional champions last season. going through the season unbeaten, and reached their second consecutive FA Youth Cup, and have also invested £5m into their academy system since the end of last season.